Journal: Frontiers in Immunology

Article Title: Pandemic Preparedness Against Influenza: DNA Vaccine for Rapid Relief

doi: 10.3389/fimmu.2021.747032

Figure Lengend Snippet: Long term antibody responses and protection after a single vaccination. Mice (n=8/group) were vaccinated i.m. with the indicated vaccines. (A–D) Serum antibodies were monitored up to 6 months after vaccination in ELISA for (A) total IgG against HA from Cal07, (B) total IgG against HA from PR8, (C) IgG1 against HA from Cal07, and (D) IgG2a against HA from Cal07. *P < 0.05 for αMHCII-HA versus Pandemrix. (E, F) At 180 days after vaccination, mice were challenged with 5xLD 50 dose of Cal07. (E) Weight was monitored. (F) Survival curve after challenge, defined by 20% weight loss. (A–E) Data shown are mean ± SEM, *P < 0.05 (two-way ANOVA) (F) *P < 0.05 (Gehan-Breslow-Wilcoxon test). n.s., not significant.

Article Snippet: Sections were then incubated with 2µg/ml rec.HA Y98F , followed by rabbit anti-HA(Cal07) pAb (11085-T54, Sino Biological, Inc) and anti-GL7-PE (144608, BioLegend, San Diego, CA, USA).

Techniques: Enzyme-linked Immunosorbent Assay

Journal: Frontiers in Immunology

Article Title: Pandemic Preparedness Against Influenza: DNA Vaccine for Rapid Relief

doi: 10.3389/fimmu.2021.747032

Figure Lengend Snippet: Rapid induction of antibodies and protection after vaccination. Mice were vaccinated i.m. with the indicated vaccines. (A–D) Serum antibodies were monitored at day 7 after vaccination in ELISA for (A) total IgG against HA from Cal07, (B) total IgG against HA from PR8, (C) IgG1 against HA from Cal07, and (D) IgG2a against HA from Cal07. Pandemrix, αMHCII-HA, and NaCl: n=16/group. AS03 and TIV: n=8/group. (E, F) At day 8 after vaccination, mice (n=8/group) were challenged with a 5xLD 50 dose of influenza Cal07. (E) Weight was monitored. (F) Survival curve, as defined by a 20% weight loss. (G, H) At day 8 after vaccination, mice (n=8/group) were challenged with a 5xLD 50 dose of influenza PR8. (G) Weight was monitored. (F) Survival curve, as defined by a 20% weight loss. (A, D, E, G) Data shown are mean ± SEM, *P < 0.05 (two-way ANOVA); (F, H) *P < 0.05 (Gehan-Breslow-Wilcoxon test). n.s., not significant.

Article Snippet: Sections were then incubated with 2µg/ml rec.HA Y98F , followed by rabbit anti-HA(Cal07) pAb (11085-T54, Sino Biological, Inc) and anti-GL7-PE (144608, BioLegend, San Diego, CA, USA).

Techniques: Enzyme-linked Immunosorbent Assay

Journal: Frontiers in Immunology

Article Title: Pandemic Preparedness Against Influenza: DNA Vaccine for Rapid Relief

doi: 10.3389/fimmu.2021.747032

Figure Lengend Snippet: Plasma cells in bone marrow and antibody avidity after vaccination. Mice (n=6/group) were vaccinated i.m. with the indicated vaccines. (A, B) Bone marrow (tibia) was harvested, and anti-HA secreting cells examined by ELISpot at (A) days 9, 14, and 21 after vaccination, or (B) day 180 post vaccination. (C) Antibody titers and avidity index of serum antibodies against HA as measured in ELISA from the indicated time points post vaccination. Data shown are mean ± SEM, *P < 0.05, **P < 0.01 (Mann-Whitney).

Article Snippet: Sections were then incubated with 2µg/ml rec.HA Y98F , followed by rabbit anti-HA(Cal07) pAb (11085-T54, Sino Biological, Inc) and anti-GL7-PE (144608, BioLegend, San Diego, CA, USA).

Techniques: Enzyme-linked Immunospot, Enzyme-linked Immunosorbent Assay, MANN-WHITNEY

Journal: Frontiers in Immunology

Article Title: Pandemic Preparedness Against Influenza: DNA Vaccine for Rapid Relief

doi: 10.3389/fimmu.2021.747032

Figure Lengend Snippet: Multifunctional T cell subsets after vaccination. Mice (n=4/group) were vaccinated once i.m. with the indicated vaccines. 21 days after vaccination, splenocytes were harvested, and following overnight resting stimulated for 4h with HA (Cal07) in the presence of a protein transport inhibitor. Single, double, or triple expression of cytokines IFNγ, IL-2, and TNFα were investigated for CD4 (A) and CD8 (B) T cell subsets. (C, D) Effector memory (T EM ) and central memory (T CM ) T cell subsets were defined as CD44 + CD64L - , and CD44 + CD64L + , respectively. (C) Presence of CD4 T EM . (D) Presence of CD8 T EM . (E) Presence of CD4 T CM . (F) Presence of CD8 T CM . Data shown are mean ± SEM, *P < 0.05, **P < 0.01, ***P < 0.001 (Anova og Tukey’s multiple comparison).

Article Snippet: Sections were then incubated with 2µg/ml rec.HA Y98F , followed by rabbit anti-HA(Cal07) pAb (11085-T54, Sino Biological, Inc) and anti-GL7-PE (144608, BioLegend, San Diego, CA, USA).

Techniques: Expressing

Journal: Immunity

Article Title: A modified vaccinia Ankara vector-based vaccine protects macaques from SARS-CoV-2 infection, immune pathology, and dysfunction in the lungs

doi: 10.1016/j.immuni.2021.02.001

Figure Lengend Snippet:

Article Snippet: SARS-CoV-2 (2019-nCoV) Spike RBD Antibody, Rabbit PAb, Antigen Affinity Purified , Sino Biologicals , Cat#40592-T62 , .

Techniques: Adsorption, Affinity Purification, Modification, Recombinant, Transfection, Peptide Microarray, Plasmid Preparation, Expressing, Software